{
  "abstract": "Monocytes are central to innate and adaptive immunity, regulating tissue homeostasis and inflammation. However, data on their steady-state biodistribution in healthy humans are limited. We developed a non-invasive method to track radiolabelled autologous monocytes. Monocytes showed distinct intravascular transit kinetics vs neutrophils, with 45 min recovery of 47% for neutrophils vs 7% for monocytes (p=0.008), indicating predominant distribution in marginated pools. In patients with psoriatic and rheumatoid arthritis but no documented lung disease, we observed preferential early retention of monocytes in the lungs (transit half-life 25.2 min vs 14.2 min; p=0.008) compared with healthy volunteers, with reduced intravascular recovery in patients with rheumatoid arthritis (4.3% vs 12%; p=0.0043). These findings provide the first direct evidence of human monocyte pulmonary transit kinetics, systemic biodistribution and fate in health and inflammatory disease, advancing understanding of in vivo monocyte biology.",
  "authors": [
    {
      "affiliations": [
        "Victor Phillip Dahdaleh Heart & Lung Research Institute, University of Cambridge, Cambridge, England, UK"
      ],
      "name": "Neda Farahi"
    },
    {
      "affiliations": [
        "Victor Phillip Dahdaleh Heart & Lung Research Institute, University of Cambridge, Cambridge, England, UK"
      ],
      "name": "Anthony YKC Ng"
    },
    {
      "affiliations": [
        "Department of Pathology, University of Cambridge, Cambridge, UK"
      ],
      "name": "Joseph Hutton"
    },
    {
      "affiliations": [
        "Department of Nuclear Medicine, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK"
      ],
      "name": "Daniel Gillett"
    },
    {
      "affiliations": [
        "Research & Development, GlaxoSmithKline, Stevenage, UK"
      ],
      "name": "Rebecca C Furze"
    },
    {
      "affiliations": [
        "Research & Development, GlaxoSmithKline, Stevenage, UK"
      ],
      "name": "Huw D Lewis"
    },
    {
      "affiliations": [
        "School of Infection and Immunity, University of Glasgow, Glasgow, UK"
      ],
      "name": "Flavia Sunzini"
    },
    {
      "affiliations": [
        "School of Infection and Immunity, University of Glasgow, Glasgow, UK"
      ],
      "name": "Richard Hohne"
    },
    {
      "affiliations": [
        "School of Infection and Immunity, University of Glasgow, Glasgow, UK"
      ],
      "name": "Alex Tate"
    },
    {
      "affiliations": [
        "Rheumatology Research Unit, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK"
      ],
      "name": "Nicholas G Shenker"
    },
    {
      "affiliations": [
        "National Heart and Lung Institute, Imperial College, London, UK"
      ],
      "name": "Edwin R Chilvers"
    },
    {
      "affiliations": [
        "School of Infection and Immunity, University of Glasgow, Glasgow, UK"
      ],
      "name": "Jonathan Cavanagh"
    },
    {
      "affiliations": [
        "Victor Phillip Dahdaleh Heart & Lung Research Institute, University of Cambridge, Cambridge, England, UK"
      ],
      "name": "Charlotte Summers"
    }
  ],
  "title": "Pulmonary monocyte kinetics in healthy volunteers and subjects with inflammatory arthritis",
  "uid": "ffd71151-2787-5bbb-a225-9bab3827c27a"
}