{
  "abstract": "The diagnosis of primary ciliary dyskinesia (PCD) can be challenging in patients with mutations in the HYDIN gene, despite using electron microscopy tomography and ciliary motion analysis. Also, mutational analysis is hindered by a paralogous copy of the gene. Because there is a subtle reduction in bending capacity, occasionally a rotatory movement, but normal beat frequencies, we assessed if the combination of these changes could be diagnostic of PCD with HYDIN gene mutations. We developed a practical predictive tool using artificial intelligence which can be used to select patients who should be evaluated in detail for HYDIN mutations.",
  "authors": [
    {
      "affiliations": [
        "Department of Pediatrics, Pisa University Hospital, Pisa, Italy"
      ],
      "name": "Massimo Pifferi"
    },
    {
      "affiliations": [
        "Department of Pediatrics, University of Verona, Verona, Italy"
      ],
      "name": "Attilio Boner"
    },
    {
      "affiliations": [
        "Department of Pediatrics, Pisa University Hospital, Pisa, Italy"
      ],
      "name": "Debora Maj"
    },
    {
      "affiliations": [
        "Department of Information Engineering, University of Pisa, Pisa, Italy"
      ],
      "name": "Alessandro Cudazzo"
    },
    {
      "affiliations": [
        "Laboratory of Molecular Genetics, Pisa University Hospital, Pisa, Italy"
      ],
      "name": "Angela Michelucci"
    },
    {
      "affiliations": [
        "Institute of Clinical Physiology National Research Council, Pisa, Italy"
      ],
      "name": "Gabriele Donzelli"
    },
    {
      "affiliations": [
        "Department of Pediatrics, Pisa University Hospital, Pisa, Italy"
      ],
      "name": "Maria Di Cicco"
    },
    {
      "affiliations": [
        "Laboratory of Cytogenetics, Pisa University Hospital, Pisa, Italy"
      ],
      "name": "Angelo Valetto"
    },
    {
      "affiliations": [
        "Department of Pediatrics, Pisa University Hospital, Pisa, Italy"
      ],
      "name": "Diego Peroni"
    },
    {
      "affiliations": [
        "Imperial College London, London, UK",
        "Brompton Hospital, London, UK"
      ],
      "name": "Andrew Bush"
    }
  ],
  "title": "Towards a practical tool to identify HYDIN genotype using high-speed videomicroscopy",
  "uid": "c8ad407b-68c9-504d-897a-a6a7415ca9ab"
}