{
  "abstract": "Lung cancer causes more deaths than any other cancer because it is usually diagnosed after spreading beyond the margins of curative treatment. Clinical trials have demonstrated a reduction in deaths from lung cancer by screening with low-dose CT scans, but applying these at scale is expensive, and the national program is therefore only available to people at the highest risk. This means that 60% people diagnosed with lung cancer would not have been eligible for a screening scan, missing out on the chance for an early diagnosis. New tests (biomarkers) are needed for early detection screening across the population. Immune cells are intimately involved in cancer evolution from the earliest stages. Given that multiple immune cells can interact with each cancer cell, signals of cancer exposure should be detectable when cancer burden is low in early-stage disease. In this study, we tested the hypothesis that lung cancer is associated with detectable differences in circulating peripheral immune cells. We recruited 17 patients with lung cancer and 32 current/former smokers without lung cancer and performed mass cytometry (30 marker panel) on whole blood, processed fresh on the day of sample donation. Lung cancer was associated with a significant increase in intermediate monocytes (p<0.0001) and a decrease in sub-populations of CD8 T cells (p<0.05). Intermediate monocyte levels could separate lung cancer from controls with a sensitivity of 94% and a specificity of 71%, with area under the curve (AUC) of 0.8989. Current work involves confirming these findings in a larger cohort of patients and identifying minimal panels of antibodies that distinguish these subset changes for prototype clinical tests.",
  "authors": [
    {
      "affiliations": [
        "University of Manchester, Manchester, UK"
      ],
      "name": "SB Knight"
    },
    {
      "affiliations": [
        "University of Manchester, Manchester, UK"
      ],
      "name": "R Hodgetts"
    },
    {
      "affiliations": [
        "University of Manchester, Manchester, UK"
      ],
      "name": "C Andrews"
    },
    {
      "affiliations": [
        "University of Manchester, Manchester, UK"
      ],
      "name": "A Blake"
    },
    {
      "affiliations": [
        "University of Manchester, Manchester, UK"
      ],
      "name": "T Hussell"
    },
    {
      "affiliations": [
        "University of Manchester, Manchester, UK"
      ],
      "name": "P Crosbie"
    },
    {
      "affiliations": [
        "Northern Care Alliance Foundation Trust, Manchester, UK"
      ],
      "name": "N Diar-Bakerly"
    },
    {
      "affiliations": [
        "University of Manchester, Manchester, UK"
      ],
      "name": "L Diar-Bakerly"
    },
    {
      "affiliations": [
        "Northern Care Alliance Foundation Trust, Manchester, UK"
      ],
      "name": "S Grundy"
    },
    {
      "affiliations": [
        "University of Manchester, Manchester, UK"
      ],
      "name": "S Kirkham"
    }
  ],
  "title": "S62 High dimensional immune analysis reveals immune subsets associated with lung cancer",
  "uid": "79ed6da2-1a65-5e96-9ded-ec0b4fec2226"
}