{
  "abstract": "Background Neuronal apoptosis is a hallmark of ischaemic stroke, yet effective neuroprotective therapies remain scarce. Recent studies suggest that Sox10-lineage cells (SOL) can transfer nuclear material to neurons. However, whether this process contributes to neuronal survival after stroke remains unknown.Methods Sox10-iCreERT2:Ai9 mice subjected to distal middle cerebral artery occlusion (dMCAO) were used to investigate SOL to neuron material transfer after ischaemic injury. High-resolution confocal imaging, AAV-based lineage tracing and single-nucleus RNA sequencing were employed to identify tdTomato-positive neurons and characterise their transcriptional profiles. Behavioural tests and histological analyses were performed to evaluate the neuroprotective effects of tamoxifen treatment.Results A subset of neurons from cortical layer 6 expressed tdTomato protein derived from SOL, suggesting intercellular material transfer. Notably, tdTomato + neurons exhibited enhanced Bcl2 expression. High-dose tamoxifen (160 mg/kg) increased the number of tdTomato+ neurons by approximately fourfold compared with low-dose treatment. Correspondingly, the proportion of apoptotic neurons (TUNEL+/NeuN+) in the high-dose tamoxifen group was reduced from 33.4% to 19.3%, and infarct volume decreased from 11.68% to 7.26% of the ipsilateral hemisphere after ischaemic stroke. Functionally, high-dose tamoxifen significantly improved sensorimotor recovery, as evidenced by faster adhesive removal (6.9 s vs 22.2 s) and reduced forelimb error rates in the grid-walk test.Conclusions We identify Sox10 lineage-associated tdTomato + neurons in the ischaemic cortex, with their prevalence higher under high-dose tamoxifen and associated with reduced neuronal loss and improved neurological outcomes. These findings suggest a potential link between Sox10 lineage-related cellular plasticity and neuroprotection.",
  "authors": [
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China"
      ],
      "name": "Maojiao Huang"
    },
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China"
      ],
      "name": "Xinye Huang"
    },
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China"
      ],
      "name": "Yunru Chen"
    },
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China"
      ],
      "name": "Zixin Zhang"
    },
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China"
      ],
      "name": "Ju Wang"
    },
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China"
      ],
      "name": "Qiao Yin"
    },
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China"
      ],
      "name": "Junjie Liu"
    },
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China"
      ],
      "name": "Fanbin Xie"
    },
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China"
      ],
      "name": "Wanting Huang"
    },
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China",
        "Department of Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang, China"
      ],
      "name": "Falei Yuan"
    },
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China",
        "Ophthalmology Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China"
      ],
      "name": "Chunyun Luo"
    },
    {
      "affiliations": [
        "Emergency Department, Guangzhou Medical University Second Affiliated Hospital, Guangzhou, Guangdong, China"
      ],
      "name": "Xiaosong He"
    }
  ],
  "title": "Sox10 glial-neuron nuclear communication mediates anti-apoptosis neuroprotection after ischaemic stroke",
  "uid": "c383c647-4ff6-56d2-844c-f3d38404e560"
}