{
  "abstract": "Objectives Patients with primary Sjögren’s disease (SjD) have an increased risk of B cell lymphoma. The aim of this study was to determine serum protein biomarkers for lymphoma development and to advance our understanding of the functional mechanisms underlying lymphomagenesis in SjD.Methods Patients with SjD and incident, current lymphoma (n=18) with serum sampled before treatment and at 6, 12 and 24 months of follow-up, and four patients sampled 1–5 years before lymphoma diagnosis (pre-lymphoma) were included. SjD without lymphoma (n=21), SjD with historical lymphoma (n=6) and healthy blood donors (n=39) served as controls. Differentially expressed proteins between groups were analysed using the Olink Target 96 Immuno-Oncology panel applying a false discovery rate (FDR) adjusted p value of 0.05. Protein-derived interferon activation scores (pIFN scores) were calculated.Results We determined 18 differentially expressed proteins in SjD with incident lymphoma compared with both SjD without lymphoma and healthy controls. Among the top upregulated proteins were TNFSF14, FGF2, IL8, CD40 and CXCL13, where CXCL13 was the only protein with decreased levels at follow-up. We also observed upregulated expression of CD40 in the SjD pre-lymphoma group compared with SjD without lymphoma and healthy controls. All SjD patient groups presented elevated pIFN scores compared with healthy controls, where SjD sampled pre-lymphoma showed the most distinct IFN activation.Conclusions We identified altered protein expression and an increased IFN system activation in SjD with incident lymphoma and pre-lymphoma. This knowledge may contribute to earlier detection of high-risk patients, identification of therapeutic targets and may ultimately improve SjD patient outcomes.",
  "authors": [
    {
      "affiliations": [
        "Department of Medical Sciences, Section of Rheumatology and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"
      ],
      "name": "Juliana Imgenberg-Kreuz"
    },
    {
      "affiliations": [
        "Department of Medical Sciences, Section of Rheumatology and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"
      ],
      "name": "Cecilia Fugmann"
    },
    {
      "affiliations": [
        "Department of Medical Sciences, Section of Rheumatology and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"
      ],
      "name": "Anna-Maja Molin"
    },
    {
      "affiliations": [
        "Department of Medical Sciences, Section of Rheumatology and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"
      ],
      "name": "Carin Backlin"
    },
    {
      "affiliations": [
        "Department of Medical Sciences, Section of Rheumatology and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"
      ],
      "name": "Alina Johansson"
    },
    {
      "affiliations": [
        "Department of Medical Sciences, Section of Rheumatology and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"
      ],
      "name": "Milica Vranic"
    },
    {
      "affiliations": [
        "Department of Immunology, Genetics and Pathology, Cancer Precision Medicine, Uppsala Universitet, Uppsala, Sweden"
      ],
      "name": "Anna Nikkarinen"
    },
    {
      "affiliations": [
        "Department of Medical Sciences, Section of Rheumatology and Science for Life Laboratory, Uppsala University, Uppsala, Sweden",
        "Department of Immunology, Genetics and Pathology, Cancer Precision Medicine, Uppsala Universitet, Uppsala, Sweden",
        "Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection/Rheumatology, Linkoping University Faculty of Medicine, Linköping, Sweden"
      ],
      "name": "The Autolymphoma Study Group"
    },
    {
      "affiliations": [
        "Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection/Rheumatology, Linkoping University Faculty of Medicine, Linköping, Sweden"
      ],
      "name": "Per Eriksson"
    },
    {
      "affiliations": [
        "Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection/Rheumatology, Linkoping University Faculty of Medicine, Linköping, Sweden"
      ],
      "name": "Christopher Sjöwall"
    },
    {
      "affiliations": [
        "Department of Medical Sciences, Section of Rheumatology and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"
      ],
      "name": "Eva Baecklund"
    },
    {
      "affiliations": [
        "Department of Medical Sciences, Section of Rheumatology and Science for Life Laboratory, Uppsala University, Uppsala, Sweden"
      ],
      "name": "Gunnel Nordmark"
    }
  ],
  "title": "Targeted proteomics identifies differentially expressed proteins in Sjögren’s disease with incident lymphoma",
  "uid": "68c413d3-a642-5b86-80cb-03f17f0c0542"
}