{
  "abstract": "Objective Epidemiological studies have already established a link between occupational agricultural exposures and numerous cancers. However, despite extensive epidemiological study, the biological plausibility of the link between these exposures and cancer development has not been definitively confirmed. Oxidative stress is one of the genotoxicity mechanisms often highlighted in pesticide toxicity mechanisms. This oxidative mechanism can produce aldehydes from lipid peroxidation. We propose here to assess oxidative damages associated with occupational activities on farms through DNA adducts and urinary oxidative biomarkers.Material and Methods Granulocytes DNA adducts formed from 8 aldehydes and urinary 8-oxo-deoxyguanosine were quantified by liquid chromatography coupled with tandem mass spectrometry in 539 samples from a French agricultural biobank including cattle breeders and open field farmers. Correlation of oxidative stress with the use of 168 pesticides declared by the farmers was also assessed.Results The samples were collected in Normandy between 1997 and 2000. Average age was 44 years. Among the participants in the cohort, 59% were men and 41% were women, 67% never smocked, 18% were former smokers and 15%. Thes results will be given only for DNA adducts formed from acetaldehyde (AAdG) and crotonaldehyde (CrotodG). The results differed between men and women, so the analyses were stratified by gender and only the results for men will be presented. Among men 55% reported using herbicides, insecticides or fungicides. Smoking status was shown to have an effect on DNA adduct levels. With regard to agricultural tasks specifically, the use of herbicides on grassland, harvesting, pea cultivation, milking, the use of preventive treatments on animals, and the cleaning and maintenance of agricultural equipment were shown to have an effect on DNA adduct levels.Conclusion This study is the first to use DNA adducts formed from aldehydes as biomarkers of effect in a cohort of agricultural workers.",
  "authors": [
    {
      "affiliations": [
        "Inserm U1086 ANTICIPE Normandie Univ University of Caen Normandie Comprehensive Cancer Center François Baclesse. PRISMM Platform, PLATON Service Unit LABÉO"
      ],
      "name": "Raphaël Delépée"
    },
    {
      "affiliations": [
        "Inserm U1086 ANTICIPE Normandie Univ University of Caen Normandie Comprehensive Cancer Center François Baclesse. PRISMM Platform, PLATON Service Unit LABÉO"
      ],
      "name": "Quentin Vandoolaeghe"
    },
    {
      "affiliations": [
        "Inserm U1086 ANTICIPE Normandie Univ University of Caen Normandie Comprehensive Cancer Center François Baclesse. PRISMM Platform, PLATON Service Unit LABÉO"
      ],
      "name": "Yolaine Guérin"
    },
    {
      "affiliations": [
        "Inserm U1086 ANTICIPE Normandie Univ University of Caen Normandie Comprehensive Cancer Center François Baclesse. PRISMM Platform, PLATON Service Unit LABÉO"
      ],
      "name": "Stéphanie Lagadu"
    },
    {
      "affiliations": [
        "Inserm U1086 ANTICIPE Normandie Univ University of Caen Normandie Comprehensive Cancer Center François Baclesse. PRISMM Platform, PLATON Service Unit LABÉO"
      ],
      "name": "Claire Lopez-Piffet"
    },
    {
      "affiliations": [
        "Inserm U1086 ANTICIPE Normandie Univ University of Caen Normandie Comprehensive Cancer Center François Baclesse. PRISMM Platform, PLATON Service Unit LABÉO"
      ],
      "name": "Valérie Bouchart"
    },
    {
      "affiliations": [
        "Inserm U1086 ANTICIPE Normandie Univ University of Caen Normandie Comprehensive Cancer Center François Baclesse. PRISMM Platform, PLATON Service Unit LABÉO"
      ],
      "name": "Pierre Lebailly"
    }
  ],
  "title": "8290591 Use of DNA adducts formed from aldehydes as biomarkers of oxidative stress effects associated with agricultural work activities",
  "uid": "b4afa8f8-13b6-56e3-9350-2b79dfcd7fa1"
}