{
  "abstract": "Objective Children from agricultural families are often para-occupationally exposed to pesticides. We associated urinary biomarkers of pesticide exposure with relative telomere length (rTL) and mtDNA copy number (mtDNA-cn) in 270 5-year-old children from agricultural families who participated in the Infants’ Environmental (ISA) birth cohort.Methods We repeatedly measured biomarkers of pesticide exposure in maternal urine during pregnancy and in children’s urine at 5-years. rTL and mtDNA-cn were determined in children’s saliva, relative to a single-copy hemoglobin beta gene, using real-time PCR. We associated log10 transformed mean urinary biomarker concentrations with log10 transformed rTL and mtDNA-cn, using separate multivariate linear regression and generalized additive models, and adjusted for possible confounders.Results Exposure to fungicides and 2,4-D was associated with changes in rTL and mtDNA-cn. Higher prenatal hydroxy-thiabendazole was associated with shorter rTL, while current 2,4-D was associated with longer rTL:%-change-in-β (95%CI) = -0.19 (-0.36, -0.02) and = 0.38 (0.01, 0.75) per tenfold increase in exposure, respectively. In addition, prenatal hydroxy-pyrimethanil was slightly associated with mtDNA-cn: β =0.33 (-0.05, 0.70) and 2,4-D was non-linearly associated with mtDNA-cn (estimated degrees of freedom of smoothed beta = 2.78, p=0.04), while current urinary ethylene thiourea (biomarker of mancozeb) and hydroxy-tebuconazole were associated with decreased and increased mtDNA-cn:%-change-in-β (95%CI) = -0.84 (1.71, 0.05) and 0.71 (0.01, 1.40), respectively. Biomarkers of pyrethroid and chlorpyrifos exposure were not associated with rTL or mtDNA-cn.Conclusions Prenatal and childhood exposure to fungicides and 2,4-D may alter rTL and mtDNA-cn, which is of concern as shorter TL may reflect an increased risk of age-related diseases. Longer TL might reflect changes in cellular aging, including increased cellular mutation risks. Finally, increased mtDNA-cn indicates compensation for oxidative stress, while decreased levels reflect oxidative stress.",
  "authors": [
    {
      "affiliations": [
        "Universidad Nacional, Heredia, Costa Rica. Division of Occupational and Environmen-tal Medicine, Lund University, Sweden. Center for Environmental Research and Community Health (CERCH) School of Public Health University of California, Berkeley. Department of Biological Sciences, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA. Center for Human Health and the Environment, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA"
      ],
      "name": "Berna van Wendel de Joode"
    },
    {
      "affiliations": [
        "Universidad Nacional, Heredia, Costa Rica. Division of Occupational and Environmen-tal Medicine, Lund University, Sweden. Center for Environmental Research and Community Health (CERCH) School of Public Health University of California, Berkeley. Department of Biological Sciences, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA. Center for Human Health and the Environment, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA"
      ],
      "name": "Christian H Lindh"
    },
    {
      "affiliations": [
        "Universidad Nacional, Heredia, Costa Rica. Division of Occupational and Environmen-tal Medicine, Lund University, Sweden. Center for Environmental Research and Community Health (CERCH) School of Public Health University of California, Berkeley. Department of Biological Sciences, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA. Center for Human Health and the Environment, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA"
      ],
      "name": "Jorge Peñaloza-Castañeda"
    },
    {
      "affiliations": [
        "Universidad Nacional, Heredia, Costa Rica. Division of Occupational and Environmen-tal Medicine, Lund University, Sweden. Center for Environmental Research and Community Health (CERCH) School of Public Health University of California, Berkeley. Department of Biological Sciences, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA. Center for Human Health and the Environment, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA"
      ],
      "name": "Ana María Mora"
    },
    {
      "affiliations": [
        "Universidad Nacional, Heredia, Costa Rica. Division of Occupational and Environmen-tal Medicine, Lund University, Sweden. Center for Environmental Research and Community Health (CERCH) School of Public Health University of California, Berkeley. Department of Biological Sciences, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA. Center for Human Health and the Environment, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA"
      ],
      "name": "Jane A Hoppin"
    },
    {
      "affiliations": [
        "Universidad Nacional, Heredia, Costa Rica. Division of Occupational and Environmen-tal Medicine, Lund University, Sweden. Center for Environmental Research and Community Health (CERCH) School of Public Health University of California, Berkeley. Department of Biological Sciences, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA. Center for Human Health and the Environment, North Carolina State University (NC State), Raleigh, North Carolina 27695, USA"
      ],
      "name": "Karin Bröberg"
    }
  ],
  "title": "8292450 Associations of biomarkers of pesticide exposure with telomere length and mtDNA copy number in 5-year old children from agricultural families in Costa Rica, results from the ISA birth cohort",
  "uid": "9a43e11d-792d-5a8e-8bc1-dfd33228e4b0"
}