{
  "abstract": "Background Inflammation drives damage in acute ischemic stroke (AIS). Here, we map temporal and molecular mechanisms of immune-vascular response in patients with AIS treated with endovascular thrombectomy (EVT) for anterior circulation large-vessel occlusion.Methods In this prospective cohort, 52 patients underwent serial peripheral blood sampling at groin puncture (Pre), catheter withdrawal (T0), and 6, 24, and 48 hours post-reperfusion. Thirteen immune and vascular players were quantified by mesoscale multiplex assays. Clinical outcomes were the modified Rankin Scale (mRS) score at 3 months and the National Institutes of Health Stroke Scale (NIHSS) at 24 hours.Results Adjusted by age, baseline Alberta Stroke Program Early CT Score (ASPECTS) and NIHSS scores, higher pre-EVT peripheral blood levels of interleukin (IL)−1β, IL-4, IL-10, and IL-13 were associated with poorer 24-hours NIHSS. Post-EVT reperfusion, IL-6 and its downstream effectors vascular cell adhesion molecule- (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) levels rose in peripheral blood over time, suggesting cerebrovascular inflammation, accompanied by the increased levels of acute-phase reactants C-reactive protein (CRP) and serum amyloid A (SAA), indicative of a systemic inflammatory engagement. In the same timeframe, interferon-gamma (IFN-γ) blood levels decreased. Adjusted by age, baseline ASPECT and NIHSS scores, and pre-thrombectomy biomarker levels, higher post-EVT levels of IL-6, VCAM-1, ICAM-1, and SAA were associated with poorer 24-hours NIHSS and unfavorable mRS 3 month outcomes, supporting an evolving immune dysregulation following AIS.Conclusion This exploratory study points to immune and vascular activation mechanisms from pre- to post-EVT, representing possible disease indicators and targets.",
  "authors": [
    {
      "affiliations": [
        "Department of Anesthesia and Critical Care Medicine, Gui de Chauliac University Hospital, Montpellier University Medical Center, Montpellier, France",
        "Institute of Functional Genomics (IGF), University of Montpellier, CNRS, INSERM, Montpellier, France"
      ],
      "name": "Francesca Rapido"
    },
    {
      "affiliations": [
        "Institute of Functional Genomics (IGF), University of Montpellier, CNRS, INSERM, Montpellier, France"
      ],
      "name": "Nicola Marchi"
    },
    {
      "affiliations": [
        "Department of Biostatistics, CHU Lille, Lille, France"
      ],
      "name": "Julien Labreuche"
    },
    {
      "affiliations": [
        "Department of Neurology, Gui de Chauliac Hospital, Montpellier University Medical Center, Montpellier, France"
      ],
      "name": "Adrien ter Schiphorst"
    },
    {
      "affiliations": [
        "Institute of Functional Genomics (IGF), University of Montpellier, CNRS, INSERM, Montpellier, France"
      ],
      "name": "Marine Blaquière"
    },
    {
      "affiliations": [
        "Institute of Functional Genomics (IGF), University of Montpellier, CNRS, INSERM, Montpellier, France"
      ],
      "name": "Frédéric de Bock"
    },
    {
      "affiliations": [
        "Department of Anesthesia and Critical Care Medicine, Gui de Chauliac University Hospital, Montpellier University Medical Center, Montpellier, France"
      ],
      "name": "Victoria Calcado"
    },
    {
      "affiliations": [
        "Department of Anesthesia and Critical Care Medicine, Gui de Chauliac University Hospital, Montpellier University Medical Center, Montpellier, France"
      ],
      "name": "Julien Fendeleur"
    },
    {
      "affiliations": [
        "Institute of Functional Genomics (IGF), University of Montpellier, CNRS, INSERM, Montpellier, France"
      ],
      "name": "Philippe Marin"
    },
    {
      "affiliations": [
        "Department of Anesthesia and Critical Care Medicine, Gui de Chauliac University Hospital, Montpellier University Medical Center, Montpellier, France"
      ],
      "name": "Pierre-François Perrigault"
    },
    {
      "affiliations": [
        "Department of Neuroradiology, Gui de Chauliac Hospital, Montpellier University Medical Center, Montpellier, France"
      ],
      "name": "Marinette Moynier"
    },
    {
      "affiliations": [
        "Department of Anesthesiology and Critical Care Medicine, Saint-Eloi Hospital, Montpellier University Medical Center, Montpellier, France",
        "PhyMedExp, INSERM, CNRS, University of Montpellier, Montpellier, France"
      ],
      "name": "Gerald Chanques"
    },
    {
      "affiliations": [
        "Institute of Functional Genomics (IGF), University of Montpellier, CNRS, INSERM, Montpellier, France",
        "Department of Neuroradiology, Gui de Chauliac Hospital, Montpellier University Medical Center, Montpellier, France"
      ],
      "name": "Vincent Costalat"
    },
    {
      "affiliations": [
        "Institute of Functional Genomics (IGF), University of Montpellier, CNRS, INSERM, Montpellier, France",
        "Department of Neuroradiology, Gui de Chauliac Hospital, Montpellier University Medical Center, Montpellier, France"
      ],
      "name": "Cyril Dargazanli"
    }
  ],
  "title": "Acute ischemic stroke and reperfusion drive molecular immune-vascular activations detectable in peripheral blood",
  "uid": "55e1afb4-b359-5e25-af37-2d4f5fccf910"
}