{
  "abstract": "Objectives Sjogren’s Syndrome (SjS), an autoimmune disorder marked by lymphocyte infiltration causing xerophthalmia and xerostomia, is classified into primary Sjogren’s syndrome (pSjS) and secondary Sjogren’s syndrome (sSjS). The ACR/EULAR 2016 criteria currently rely on anti-Ro/SSA antibodies, exhibiting a 50-70% positivity rate in patients with SjS. This study seeks to pinpoint a high-positivity autoantibody as a diagnostic biomarker of SjS.Methods Through protein microarray analysis, anti-lysosomal protein transmembrane 4 alpha (LAPTM4A) was identified as a potential biomarker of SS. Using the Baculovirus Expression Vector System, GST-fused LAPTM4A was generated. Enzyme-linked immunosorbent assay measured anti-LAPTM4A antibody titers in pSS (n=46), sSS (n=36), total SS (tSS, n=82), and normal controls (NCs, n=30). T-test analysis assessed and the positivity rate was calculated.Results Anti-LAPTM4A exhibited a substantial 67% positivity in pSjS via protein microarray analysis. ELISA measured anti-LAPTM4A antibody titers: pSjS (48.1 arbitrary unit(AU)), sSjS (47.54 AU), tSjS (47.85 AU), and NCs (36.02 AU). Anti-LAPTM4A antibody detection was notably elevated in pSjS, sSjS and tSjS (p<0.05). The positivity rates for anti-LAPTM4A antibody in patients negative for the anti-Ro antibody were 30%, 48%, and 43% in pSjS, sSjS, and tSjS groups, respectively.Conclusions This study aimed to evaluate the potential of the anti-LAPTM4A antibody as an additional biomarker for SjS. Elevated anti-LAPTM4A antibody titers observed in pSjS, sSjS, and tSjS compared to NCs indicate its promise as a supplementary diagnostic biomarker for SjS.",
  "authors": [
    {
      "affiliations": [
        "Ajou University School of Medicine, Suwon, South Korea"
      ],
      "name": "Chang-Hee Suh"
    },
    {
      "affiliations": [
        "Ajou University School of Medicine, Suwon, South Korea"
      ],
      "name": "Wook-Young Baek"
    },
    {
      "affiliations": [
        "Ajou University School of Medicine, Suwon, South Korea"
      ],
      "name": "Ho-Chul Kang"
    }
  ],
  "title": "PO:02:033 Anti-lysosomal protein transmembrane 4 alpha antibody as a supplementary biomaker for Sjogren’s syndrome",
  "uid": "d6f58dee-f60e-564e-b66c-6df0a2106e6a"
}