{
  "abstract": "Background Endotoxaemia without infection in lupus is mentioned with the inconclusive clinical importance.Methods With endotoxaemia and lupus activity (Systemic Lupus Erythematosus Disease Activity Index 2000 score), 46 patients with childhood-onset lupus were categorised into active lupus with endotoxaemia (n=14), inactive lupus with endotoxaemia (n=10), active lupus without endotoxaemia (n=10) and inactive lupus without endotoxaemia (n=12). The routine parameters (serum creatinine, urine sediments, proteinuria, complement, haematological aspects and histological activity index) were analysed with lupus activity and other parameters.Results Serum cytokines (tumour necrosis factor (TNF)-α, interleukin (IL)-6, IL-8 and IL-10), serum citrullinated histone H3, cell-free DNA and bacterial-free DNA were not different among groups. The extracellular traps (ETs) in the peripheral blood mononuclear cell (PBMC) fraction, measured by immunofluorescence of myeloperoxidase (MPO) and neutrophil elastase (NE), were elevated in endotoxaemia regardless of lupus disease activity. Interestingly, low-density granulocytes (LDGs), the neutrophils in the PBMC fraction after gradient separation, were elevated in active lupus regardless of endotoxaemia but higher in the patients with positive endotoxaemia. Because endotoxaemia might be derived from the gut, the blood microbiome was measured, and the Burkholderia group was the representative bacteria in active lupus with endotoxaemia. The incubation of LPS or bacterial-free DNA with neutrophils from the healthy control altered these regular-density neutrophils to LDGs.Conclusion Endotoxaemia presented in both active and inactive lupus (possibly correlated with some bacterial groups in the gut) that caused ETs in the PBMC fraction and LDGs. However, elevated LDGs were most prominent in endotoxaemia with active lupus.",
  "authors": [
    {
      "affiliations": [
        "Center of Excellence on Translational Research in Inflammation and Immunology (CETRII), Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand",
        "Interdisciplinary Program of Biomedical Sciences, Graduate School, Chulalongkorn University, Bangkok, Thailand"
      ],
      "name": "Wilasinee Saisorn"
    },
    {
      "affiliations": [
        "Center of Excellence on Translational Research in Inflammation and Immunology (CETRII), Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand"
      ],
      "name": "Pornpimol Phuengmaung"
    },
    {
      "affiliations": [
        "Division of Nephrology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand"
      ],
      "name": "Chanunya Santiworakul"
    },
    {
      "affiliations": [
        "Department of Emergency and Critical Care Medicine, The University of Tokyo, Bunkyo, Tokyo, Japan"
      ],
      "name": "Kent Doi"
    },
    {
      "affiliations": [
        "Division of Nephrology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand"
      ],
      "name": "Pornpimol Rianthavorn"
    },
    {
      "affiliations": [
        "Center of Excellence on Translational Research in Inflammation and Immunology (CETRII), Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand",
        "Division of Nephrology, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand"
      ],
      "name": "Asada Leelahavanichkul"
    }
  ],
  "title": "Endotoxaemia in childhood-onset systemic lupus erythematosus induces low-density granulocytes and extracellular traps of innate immune cells",
  "uid": "ebced4ce-0f4d-56a5-98e6-ef0a2b802792"
}