{
  "abstract": "Background Peritoneal metastasis (PM) from gastric cancer (GC) is associated with poor prognosis and limited treatment options. We investigated a novel peritoneal-targeted therapy using CF33-human sodium iodide symporter (hNIS), a chimeric orthopoxvirus, combined with anti-PD-L1 immune checkpoint blockade in an immunocompetent mouse model of GCPM.Methods We evaluated replication and cytotoxicity of CF33-hNIS in human and murine GC cell lines. We assessed a syngeneic mouse model of PM using the transgenic mouse GC ACKPY3944 cells to test the efficacy of intraperitoneal (IP) CF33-hNIS alone and combined with intravenous or IP anti-PD-L1. Next, we performed flow cytometry and immunohistochemistry to analyze immune cell populations of CD3 + T cell subsets in the peritoneal cavity and tumor microenvironment. Mice that showed complete tumor regression (CTR) were rechallenged with ACKPY3944 cells to assess memory T cell responses.Results CF33-hNIS efficiently infected and killed GC cells. In vivo, IP CF33-hNIS alone significantly prolonged survival and increased infiltration of CD3 + and CD8+ T cells within the peritoneal cavity and solid tumor. The most pronounced therapeutic effect was observed with a single high-dose of CF33-hNIS (108 plaque-forming units) combined with IP anti-PD-L1 (Combo 2) with 75% CTR. Notably, mice with CTR rejected tumor rechallenge, exhibiting significantly elevated effector and central memory T cell populations in the peritoneal cavity and spleen. IP CF33-hNIS demonstrates robust anti-tumor efficacy against GCPM, particularly when combined with IP anti-PD-L1 in the high-dose Combo 2 regimen.Conclusions These findings support a simplified, high-dose IP treatment strategy to overcome immune resistance in GCPM and provide a strong rationale for future clinical evaluation.",
  "authors": [
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Annie Yang"
    },
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Zhifang Zhang"
    },
    {
      "affiliations": [
        "Cancer Immunotherapeutics Program, Beckman Research Institute, City of Hope National Medical Center, Duarte, CA, USA"
      ],
      "name": "Anthony K Park"
    },
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Shyambabu Chaurasiya"
    },
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Sang-In Kim"
    },
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Jianming Lu"
    },
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Yoya Vashi"
    },
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Jennifer Cillis"
    },
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Hannah Valencia"
    },
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Courtney Chen"
    },
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Supriya Deshpande"
    },
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Yuman Fong"
    },
    {
      "affiliations": [
        "Depratment of Surgery, City of Hope National Medical Center, Duarte, California, USA"
      ],
      "name": "Yanghee Woo"
    }
  ],
  "title": "Intraperitoneal CF33-hNIS combined with PD-L1 blockade eradicates gastric cancer peritoneal metastases and prevents recurrence via durable T cell memory",
  "uid": "84bb80d2-1b17-5978-b9f8-ad19b05e3cf1"
}