{
  "abstract": "Multiplex immunofluorescence and immunohistochemistry (mIF/IHC) are increasingly employed antibody-based technologies that use tissue sparingly and facilitate the detection of co-localized or neighboring biomarkers. Specifically, these platforms enable spatial analyses of the tumor microenvironment as well as extended applications, for example, describing normal tissue anatomy, autoimmunity, infectious diseases, etc. mIF/IHC has greatly enhanced biomarker discovery efforts, and a growing number of studies suggest superiority to traditional IHC. Standardization of staining approaches, reporting of image analysis strategies and resultant data is critical for facilitating cross-study comparisons, validation, deployment, and generalization of findings. To address this challenge, The Society for Immunotherapy of Cancer (SITC) previously published two articles providing best practice guidelines for mIF/IHC staining, image analysis, and data sharing. Here, SITC convened stakeholders to develop the third article in the series, a consensus checklist for scientific reporting of mIF/IHC data to support and complement the best practice guidelines. The checklist includes critical components of mIF/IHC applications to be defined within publications such as detailed descriptions of analytical validation; image acquisition, selection, and registration methods; and cell clustering and spatial analysis strategies, amongst others. Such information will help with data reproducibility and comparison across studies towards future drug and assay development.",
  "authors": [
    {
      "affiliations": [
        "HAS Consulting, Miami, Florida, USA"
      ],
      "name": "Sam Sater"
    },
    {
      "affiliations": [
        "Providence Molecular Genomics Laboratory, Portland, Oregon, USA",
        "Earle A Chiles Research Institute, Portland, Oregon, USA"
      ],
      "name": "Carlo B Bifulco"
    },
    {
      "affiliations": [
        "Daiichi Sankyo Inc, Basking Ridge, New Jersey, USA"
      ],
      "name": "Jaime Rodriguez-Canales"
    },
    {
      "affiliations": [
        "Department of Anatomical Pathology, Singapore General Hospital, Singapore",
        "Institute of Molecular and Cell Biology (IMCB), A*STAR, Singapore"
      ],
      "name": "Joe Yeong"
    },
    {
      "affiliations": [
        "Merck & Co Inc, Kenilworth, New Jersey, USA"
      ],
      "name": "Guray Akturk"
    },
    {
      "affiliations": [
        "Department of Pathology, Stanford University, Stanford, California, USA"
      ],
      "name": "Michael Angelo"
    },
    {
      "affiliations": [
        "Bristol Myers Squibb, San Diego, California, USA"
      ],
      "name": "Carmen Ballesteros-Merino"
    },
    {
      "affiliations": [
        "Edinburgh Pathology, Centre for Genomic & Experimental Medicine and CRUK Scotland Centre, Institute of Genetics and Cancer, The University of Edinburgh, Edinburgh, UK"
      ],
      "name": "Peter Bankhead"
    },
    {
      "affiliations": [
        "Navinci Diagnostics, Uppsala, Sweden"
      ],
      "name": "Subham Basu"
    },
    {
      "affiliations": [
        "AstraZeneca, Gaithersburg, Maryland, USA"
      ],
      "name": "Jorge M Blando"
    },
    {
      "affiliations": [
        "Lunaphore Technologies, Tolochenaz, Switzerland"
      ],
      "name": "Saska Brajkovic"
    },
    {
      "affiliations": [
        "Lunaphore Technologies, Tolochenaz, Switzerland"
      ],
      "name": "Marco Cassano"
    },
    {
      "affiliations": [
        "Bristol Myers Squibb, Cambridge, Massachusetts, USA"
      ],
      "name": "Benjamin J Chen"
    },
    {
      "affiliations": [
        "Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA",
        "Emory University Winship Cancer Institute, Atlanta, Georgia, USA"
      ],
      "name": "Ahmet F Coskun"
    },
    {
      "affiliations": [
        "Department of Pathology and Molecular Medicine, Queen’s University, Kingston, Ontario, Canada"
      ],
      "name": "Tricia R Cottrell"
    },
    {
      "affiliations": [
        "Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain",
        "Patología, Clinica Universidad de Navarra, Pamplona, Spain"
      ],
      "name": "Carlos E De Andrea"
    },
    {
      "affiliations": [
        "Robin H. Edwards Consulting, LLC, Newtown, Pennsylvania, USA"
      ],
      "name": "Robin H Edwards"
    },
    {
      "affiliations": [
        "Department of Immuno-Oncology, Beckman Research Institute, City of Hope, Duarte, California, USA"
      ],
      "name": "Colt Egelston"
    },
    {
      "affiliations": [
        "Department of Dermatology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"
      ],
      "name": "Logan L Engle"
    },
    {
      "affiliations": [
        "Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, Maryland, USA"
      ],
      "name": "Marc S Ernstoff"
    },
    {
      "affiliations": [
        "Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA"
      ],
      "name": "Rong Fan"
    },
    {
      "affiliations": [
        "Department of Pathology, Indiana University School of Medicine, Indianapolis, Indiana, USA"
      ],
      "name": "Michael Feldman"
    },
    {
      "affiliations": [
        "Laboratory of Molecular and Tumor Immunology, Earle A Chiles Research Institute, Portland, Oregon, USA",
        "Providence Cancer Institute, Portland, Oregon, USA"
      ],
      "name": "Bernard A Fox"
    },
    {
      "affiliations": [
        "INSERM, Equipe Labellisée Ligue Contre le Cancer, Centre de Recherche des Cordeliers, Sorbonne Université, Université Paris Cité, Paris, France"
      ],
      "name": "Jerome Galon"
    },
    {
      "affiliations": [
        "Department of Oncology, Division of Pediatric Oncology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"
      ],
      "name": "Robyn Gartrell"
    },
    {
      "affiliations": [
        "Department of Immunology and Immunotherapy, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, New York, USA"
      ],
      "name": "Sacha Gnjatic"
    },
    {
      "affiliations": [
        "The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"
      ],
      "name": "Benjamin F Green"
    },
    {
      "affiliations": [
        "Center for Immuno-Oncology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA"
      ],
      "name": "James L Gulley, MD,"
    },
    {
      "affiliations": [
        "Indica Labs, Albuquerque, New Mexico, USA"
      ],
      "name": "Anne Hellebust"
    },
    {
      "affiliations": [
        "Laboratory of Pathology, National Institutes of Health, Bethesda, Maryland, USA"
      ],
      "name": "Stephen Hewitt"
    },
    {
      "affiliations": [
        "Bristol Myers Squibb, Princeton, New Jersey, USA"
      ],
      "name": "Travis J Hollmann"
    },
    {
      "affiliations": [
        "Center for Immuno-Oncology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA"
      ],
      "name": "Lucas A Horn"
    },
    {
      "affiliations": [
        "Abcam, Cambridge, England, UK"
      ],
      "name": "William J Howat"
    },
    {
      "affiliations": [
        "Quanterix Corp, Billerica, Massachusetts, USA"
      ],
      "name": "Clifford C Hoyt"
    },
    {
      "affiliations": [
        "Laboratory of Molecular and Tumor Immunology, Earle A Chiles Research Institute, Portland, Oregon, USA",
        "Providence Cancer Institute, Portland, Oregon, USA"
      ],
      "name": "Shawn M Jensen"
    },
    {
      "affiliations": [
        "Frazer Institute, Faculty of Medicine, The University of Queensland, Brisbane, Queensland, Australia"
      ],
      "name": "Arutha Kulasinghe"
    },
    {
      "affiliations": [
        "Center for Immuno-Oncology, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, USA"
      ],
      "name": "Wiem Lassoued"
    },
    {
      "affiliations": [
        "Bruker Spatial Biology, San Jose, California, USA"
      ],
      "name": "Steven Lott"
    },
    {
      "affiliations": [
        "Standard BioTools, Markhan, ON, Canada"
      ],
      "name": "James Mansfield"
    },
    {
      "affiliations": [
        "Histology, Research Center Borstel - Leibniz Lung Center, Borstel, Germany",
        "German Center for Lung Research (DZL), Borstel, Germany"
      ],
      "name": "Sebastian Marwitz"
    },
    {
      "affiliations": [
        "University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA"
      ],
      "name": "George Netto"
    },
    {
      "affiliations": [
        "Earle A Chiles Research Institute, Portland, Oregon, USA"
      ],
      "name": "David B Page"
    },
    {
      "affiliations": [
        "Department of Translational Medicine & Clinical Pharmacology, Boehringer Ingelheim Pharmaceuticals Inc, Ridgefield, Connecticut, USA"
      ],
      "name": "Edwin Parra"
    },
    {
      "affiliations": [
        "Departments of Pathology and Medicine (Oncology), Yale School of Medicine, New Haven, Connecticut, USA"
      ],
      "name": "David L Rimm"
    },
    {
      "affiliations": [
        "Brigham and Women’s Hospital, Boston, Massachusetts, USA"
      ],
      "name": "Scott J Rodig"
    },
    {
      "affiliations": [
        "Department of Pathology, GasthuisZusters Antwerpen, Antwerp, Belgium",
        "Division of Research, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia"
      ],
      "name": "Roberto Salgado"
    },
    {
      "affiliations": [
        "Institute for Computational Biomedicine, Heidelberg University, Heidelberg, Germany",
        "Faculty of Medicine, Institute of Pathology, Heidelberg University Hospital, Heidelberg, Germany",
        "Translational Spatial Profiling Center (TSPC), Heidelberg, Germany"
      ],
      "name": "Denis Schapiro"
    },
    {
      "affiliations": [
        "Departments of Pathology and Medicine (Oncology), Yale School of Medicine, New Haven, Connecticut, USA"
      ],
      "name": "Kurt A Schalper, MD,"
    },
    {
      "affiliations": [
        "Division of Dermatopathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"
      ],
      "name": "Joel C Sunshine"
    },
    {
      "affiliations": [
        "AstraZeneca, Gaithersburg, Maryland, USA"
      ],
      "name": "Michael J Surace"
    },
    {
      "affiliations": [
        "Department of Astronomy and Physics, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA",
        "The Mark Foundation Center for Advanced Genomics and Imaging at Johns Hopkins University, Baltimore, Maryland, USA"
      ],
      "name": "Alexander S Szalay"
    },
    {
      "affiliations": [
        "Cancer Diagnosis Program, National Cancer Institute, Bethesda, Maryland, USA"
      ],
      "name": "Magdalena Thurin"
    },
    {
      "affiliations": [
        "Division of Hematology, Mayo Clinic, Rochester, Minnesota, USA"
      ],
      "name": "Jose C Villasboas"
    },
    {
      "affiliations": [
        "Ultivue, Inc, Cambridge, Massachusetts, USA",
        "Roche Diagnostics Solutions, Oro Valley, Arizona, USA"
      ],
      "name": "Keith Wharton"
    },
    {
      "affiliations": [
        "Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"
      ],
      "name": "Ignacio I Wistuba"
    },
    {
      "affiliations": [
        "Merck, Rahway, New Jersey, USA"
      ],
      "name": "Jennifer H Yearley"
    },
    {
      "affiliations": [
        "Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"
      ],
      "name": "Yinyin Yuan"
    },
    {
      "affiliations": [
        "Biomedical Informatics and Data Science Directorate, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA"
      ],
      "name": "Geroge Zaki"
    },
    {
      "affiliations": [
        "Genentech, South San Francisco, California, USA"
      ],
      "name": "James Ziai"
    },
    {
      "affiliations": [
        "Division of Dermatopathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"
      ],
      "name": "Janis M Taube"
    }
  ],
  "title": "Society for Immunotherapy of Cancer: Standards for Reporting of Multiplex Immunohistochemistry/Immunofluorescence Assays (STORMI)",
  "uid": "df60a742-1136-5ea8-89f2-05567b0503d8"
}