{
  "abstract": "Background Enhancing immunogenicity and antigen-presentation efficiency is critical for tumor vaccine development. While yeast-surface glycoprotein side chains can improve antigen presentation, their ability to deliver tumor antigens remains limited.Methods The composite carbon nanopolymers (Asparagine-N-acetylglucosamine-Mannose derived carbon nanopolymers, ANM-NPs) mimicking yeast-surface glycans side chains were prepared using asparagine, N-acetylglucosamine and mannose as precursors via microwave-assisted synthesis method. Combined with whole-cell tumor antigen (T-Ag), the vaccine ANM-NPs@T-Ag was prepared. The expression levels of major histocompatibility complex-II, CD80, and CD86 were analyzed by flow cytometry to evaluate the enhancement of antigen presentation. The antitumor efficacy of ANM-NPs@T-Ag was assessed by H22-LUC and B16-F10-LUC tumor-bearing models.Results ANM-NPs have an average particle size of approximately 21.69 nm and exhibit good stability. Compared with traditional aluminum adjuvants, ANM-NPs demonstrate superior efficacy in promoting antigen presentation and immune activation. ANM-NPs effectively stimulated innate immunity, facilitated immune cell infiltration, and enhanced adaptive immune responses. The vaccine ANM-NPs@T-Ag targeted dendritic cells via mannose receptors, activating the lectin pathway, improving complement opsonization, and enhancing antigen presentation. ANM-NPs@T-Ag stimulated cellular/humoral immunity, increased tumor-specific IgG, and inhibited tumor growth. ANM-NPs@T-Ag also enhanced the antitumor effect of α-PD-1 (programmed cell death protein-1).Conclusion These findings demonstrate that ANM-NPs can serve as a potential vaccine adjuvant, and ANM-NPs@T-Ag represents a promising tumor vaccine candidate. Cite Now",
  "authors": [
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China"
      ],
      "name": "Zhuangzhuang Feng"
    },
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China"
      ],
      "name": "Xinxiu Shi"
    },
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China"
      ],
      "name": "Zhiyang Li"
    },
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China"
      ],
      "name": "Minming Lou"
    },
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China"
      ],
      "name": "Bijia Cui"
    },
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China"
      ],
      "name": "Mengzhan Chen"
    },
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China"
      ],
      "name": "Lei Zhang"
    },
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China"
      ],
      "name": "Yujie Sun"
    },
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China"
      ],
      "name": "Heng Zhang"
    },
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China"
      ],
      "name": "Yi-nan Li"
    },
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China",
        "Department of Dermatovenereology, Tianjin Institute of Sexually Transmitted Disease, Tianjin Medical University General Hospital, Tianjin, China"
      ],
      "name": "Huijuan Liu"
    },
    {
      "affiliations": [
        "State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, China"
      ],
      "name": "Tao Sun"
    }
  ],
  "title": "Biomimetic carbon nanopolymers ANM-NPs act on mannose receptors and complement receptors to promote tumor antigen presentation",
  "uid": "47296c30-6898-55c3-aa9e-cb4460925251"
}