{
  "abstract": "Background Colorectal cancer presents high risks of recurrence and distant metastasis, primarily due to therapeutic resistance and the challenges associated with detecting secondary lesions. While photothermal therapy (PTT) offers a promising method for localized tumor ablation and may partially overcome drug resistance, its ability to induce systemic antitumor immunity—specifically the abscopal effect—remains limited. Enhancing the abscopal effect requires integration with immune-stimulatory approaches. Interferon-β (IFN-β), a cytokine known for both direct cytotoxicity and immune regulation, represents a strong candidate for such combination strategies.Methods To achieve tumor-targeted delivery, we utilized polydopamine nanoparticles conjugated with an anti-Claudin-3 (CLDN3) antibody for PTT and applied a fusion cytokine (ABN202), consisting of IFN-β linked to the same antibody, to further promote immune activation. Using a bilateral colorectal cancer model in human IFNAR1/2 knock-in mice, we assessed the therapeutic impact of the combination on both local and distant tumors. Immune profiling was conducted using flow cytometry, with a focus on dendritic cell activation, DAMPs release, and memory T cell differentiation.Results The combined therapy produced effectively inhibited growth at distal, untreated tumor locations. Mechanistically, this synergy was associated with increased release of damage-associated molecular patterns and elevated dendritic cell activation. Importantly, we observed a significant increase in CD8 + central (Tcm) and effector memory (Tem) T cells in immune cell following treatment. Upon tumor re-challenge, mice maintained long-term protection, indicative of durable immune memory formation.Conclusions This study provides evidence that combining IFN-β with CLDN3-targeted PTT potentiates systemic antitumor immunity and fosters long-term protection against recurrence. These results highlight a translationally promising strategy for treating metastatic colorectal cancer by converting localized tumor destruction into robust, memory-driven immune responses.",
  "authors": [
    {
      "affiliations": [
        "Seoul National University, Seoul, Republic of Korea",
        "Seoul National University, Seoul, Gwanak-gu, Republic of Korea"
      ],
      "name": "Myeung Ryun Seo"
    },
    {
      "affiliations": [
        "Seoul National University, Seoul, Gwanak-gu, Republic of Korea"
      ],
      "name": "Jinwon Park"
    },
    {
      "affiliations": [
        "ABION Inc., Seoul, Gwanak-gu, Republic of Korea"
      ],
      "name": "Heegeon Park"
    },
    {
      "affiliations": [
        "Seoul National University, Seoul, Gwanak-gu, Republic of Korea"
      ],
      "name": "Sang Moo Lee course"
    },
    {
      "affiliations": [
        "ABION Inc., Seoul, Gwanak-gu, Republic of Korea"
      ],
      "name": "Saehyung Lee"
    },
    {
      "affiliations": [
        "Kyungsung University, Busan, Nam-gu, Republic of Korea"
      ],
      "name": "Hobin Yang"
    },
    {
      "affiliations": [
        "Seoul National University, Seoul, Gwanak-gu, Republic of Korea"
      ],
      "name": "Sungyoul Hong"
    },
    {
      "affiliations": [
        "Seoul National University, Seoul, Gwanak-gu, Republic of Korea"
      ],
      "name": "Yu-Kyoung Oh"
    },
    {
      "affiliations": [
        "Seoul National University, Seoul, Gwanak-gu, Republic of Korea"
      ],
      "name": "Youngkee Shin"
    }
  ],
  "title": "698 Enhancing abscopal effects in colorectal cancer via photothermal therapy combined with Interferon-β",
  "uid": "c9c399bf-4bff-597a-8def-28d79a3c8427"
}