{
  "abstract": "Background CD70, the ligand of CD27, is a co-stimulatory molecule involved in T-cell priming and survival. Aberrant expression of CD70 has been observed in various malignancies and is implicated in immune evasion, tumor progression, and resistance to checkpoint blockade. Several CD70-targeted therapies are under active clinical development, but the immunologic context of CD70 expression across cancers remains poorly understood. A comprehensive characterization of CD70 within the tumor immune microenvironment (TIME) could inform therapeutic strategies and patient selection. In this study, we broadly evaluate the relationship between CD70 transcript abundance and immune cell infiltration across diverse tumor types.Methods Bulk RNA sequencing data from The Cancer Genome Atlas (TCGA) Pan-Cancer cohort (n > 9,000) were analyzed. CD70 expression levels were quantified in transcripts per million (TPM). Immune infiltration scores for 64 distinct immune and stromal cell types were estimated using the xCell algorithm, which infers cell type abundances from transcriptomic data. Spearman correlation coefficients (ρ) were computed between CD70 expression and each immune cell score. Immune cell types with absolute ρ > 0.2 were considered biologically meaningful. The analysis was performed across all tumor types without restricting by histology, ensuring pan-cancer generalizability.Results CD70 expression showed consistent positive correlations with a broad range of immune cell subsets. The strongest associations were observed with activated dendritic cells (aDC; ρ = 0.42), mesangial cells (ρ = 0.38), conventional dendritic cells (DC; ρ = 0.35), macrophages (ρ = 0.34), and monocytes (ρ = 0.34). CD4+ naive T-cells (ρ = 0.32) and CD8+ central memory T-cells (ρ = 0.25) also showed significant positive correlations. Sixteen immune cell types exhibited ρ > 0.2, encompassing primarily myeloid and T-cell lineages ( figure 1). No strong negative correlations were detected. These findings suggest that CD70 expression is enriched in immune-infiltrated TMEs across tumor types.Conclusions This comprehensive pan-cancer analysis demonstrates that CD70 expression is tightly associated with elevated myeloid and T-cell infiltration, particularly dendritic cells and CD8+ T-cell subsets. CD70 may thus serve as a biomarker of inflamed TIMEs and holds promise as a therapeutic target in immuno-oncology. Further validation using spatial profiling and correlation with clinical outcomes is warranted to establish CD70’s predictive value for response to immunotherapy and its utility in patient stratification.Abstract 152 Figure 1CD70 expression (TPM) vs. Immune Infiltration (xCell) (Immune Cell Types with |ρ| > 0.2). Correlation between CD70 expression (TPM) and immune cell infiltration (xCell) across TCGA tumors",
  "authors": [
    {
      "affiliations": [
        "Sentara Albemarle Medical Center, USACS, Elizabeth City, NC, USA"
      ],
      "name": "Mobina Shrestha"
    }
  ],
  "title": "152 CD70 expression correlates with myeloid and T-cell infiltration across cancers: a pan cancer transcriptomic deconvolution study",
  "uid": "4365d115-1758-52cb-8179-662e9001d25f"
}