{
  "abstract": "Myeloproliferative neoplasms (MPNs) are clonal disorders characterised by excessive proliferation of myeloid lineages. Mutations in MPL exon 10, including p.Trp515Leu and p.Trp515Lys, are recurrent and clinically relevant. 1 2 Sanger sequencing is traditionally used for DNA sequencing3 but has limited sensitivity, typically detecting variants at approximately 15%–20% variant allele frequency (VAF) in routine practice.4",
  "authors": [
    {
      "affiliations": [
        "Department of Laboratory Medicine, Molecular Diagnosis Centre, National University Health System, Singapore"
      ],
      "name": "Dilys Lau"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine, Molecular Diagnosis Centre, National University Health System, Singapore"
      ],
      "name": "Janice Yen Qi Loo"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine, Molecular Diagnosis Centre, National University Health System, Singapore"
      ],
      "name": "Sau-Yoke Ng"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine, Molecular Diagnosis Centre, National University Health System, Singapore"
      ],
      "name": "Dan Thu Van"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine, Molecular Diagnosis Centre, National University Health System, Singapore"
      ],
      "name": "Wei Cheng David Kuek"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine, Molecular Diagnosis Centre, National University Health System, Singapore"
      ],
      "name": "Chean Nee Chai"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine, Molecular Diagnosis Centre, National University Health System, Singapore"
      ],
      "name": "Joanna Kia Min Tan"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine, Molecular Diagnosis Centre, National University Health System, Singapore"
      ],
      "name": "Chun Kiat Lee"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine, Molecular Diagnosis Centre, National University Health System, Singapore"
      ],
      "name": "Benedict Junrong Yan"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine, Molecular Diagnosis Centre, National University Health System, Singapore"
      ],
      "name": "Tim Hon Man Chan"
    }
  ],
  "title": "Validation of polymerase chain reaction-indexed next-generation sequencing assay on the Illumina iSeq 100 platform for MPL exon 10 mutation detection: a robust alternative to Sanger sequencing",
  "uid": "813f3b8c-f62e-58f6-a55b-508268c82f66"
}