{
  "abstract": "Aims To determine if the frequency of CD103+ T cells among background lymphocytes is associated with non-haematopoietic metastasis in samples of clinically concerning unexplained lymphadenopathy.Methods We retrospectively reviewed clinical flow cytometry data from 126 lymph node (LN) samples including 36 involved by metastatic non-haematopoietic malignancy and 90 control LNs (54 benign/reactive and 36 lymphomas). We evaluated the frequency of CD103+ cells among CD3+, CD3+CD4+ and CD3+CD8+ T cell subsets. Immunohistochemistry was used to visualise CD103 expression in a limited number of cases.Results CD103 expression in ≥3% of total CD3+ T cells and ≥7% of CD3+CD8+ T cells correlated with the presence of metastatic non-haematopoietic malignancy in LNs, and differentiated LNs with metastasis from control LNs with sensitivities of 75% (95% CI 59% to 86%) and 78% (95% CI 62% to 88%) and specificities of 100% (95% CI 96% to 100%) and 99% (95% CI 94% to 100%), respectively.Conclusion Increased frequency of CD103+ T cells is associated with metastatic non-haematopoietic malignancies in excisional and core needle biopsies of clinically concerning lymphadenopathy.",
  "authors": [
    {
      "affiliations": [
        "Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada"
      ],
      "name": "Mahboobsadat Hamidi"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA"
      ],
      "name": "Jason E Love"
    },
    {
      "affiliations": [
        "Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada"
      ],
      "name": "Parham Minoo"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA"
      ],
      "name": "Afshin Shameli"
    }
  ],
  "title": "Increased CD103+ T lymphocytes in lymph nodes as a marker of metastasis from non-haematopoietic malignancies",
  "uid": "520b5b89-50fb-515a-b2d2-4d6725d8f685"
}