{
  "abstract": "Background Vascular dysfunction is common in patients with diabetes mellitus and increases the risk of morbidity and mortality associated with cardiovascular complications. Although our understanding of the molecular mechanisms responsible is incomplete, it is well established that poor glycaemic control plays a critical role, especially with reference to type 2 diabetes (T2DM). In many conditions associated with vascular dysfunction a narrowing of blood vessels and an increase in platelet activation, is observed.Aims We hypothesise that GV will impact the expression and function of key proteins that regulate vascular tone and motility and platelet activation, and impact cell-to cell interactions between endothelial cells, smooth muscle cells and platelets via changes in the production and molecular profile of extracellular vesicles (EVs).Methods Human umbilical vein endothelial cells (HUVECs) were cultured in medium with variable glucose concentrations. Immunoblotting of cell lysates and detection of protein expression was performed. EV’s are quantified from medium removed from cells by FACS analysis, nanoparticle tracking analysis and transmission electron microscopy.Conclusions Data shows a decrease in endothelial Nitric Oxide synthase (eNOS) and phospho-eNOS expression and increase in Prostaglandin I synthase expression in HUVECs culture under hypoglycaemic (2 mmol), hyperglycaemic (20 mmol) and variable glucose (alternating 2-20mmol every 24 hours) compared to normal glucose (5.6 mmol) conditions ( figures 1 and 2). We will present additional data showing how these changes impact the release of prostacyclin and thromboxane A2 and EV release from ECs.Abstract 525 Figure 1Expression of eNOS in HUVECs under different glucose conditionsAbstract 525 Figure 2Expression of PGIS in HUVECs under different glucose conditions",
  "authors": [
    {
      "affiliations": [
        "Hull York Medical School, Hull, United Kingdom"
      ],
      "name": "Laura Offler"
    },
    {
      "affiliations": [
        "Hull York Medical School, Hull, United Kingdom"
      ],
      "name": "Timothy Palmer"
    }
  ],
  "title": "525 The impact of glycaemic variation on vascular endothelial cell function",
  "uid": "777fd0ab-0821-572a-abd5-23b03ad88c0e"
}