{
  "abstract": "In patients with atherosclerotic cardiovascular disease (ASCVD) lowering LDL cholesterol (LDL-C) reduces the risk of adverse cardiovascular events. However, these patients remain at continued risk of adverse cardiovascular events despite achieving optimal LDL-C lowering. We hypothesised that this ‘residual risk’ is mediated by epigenetic programming of haematopoietic stem cells (HSC) which persists after cholesterol levels are lowered and results in sustained effects on innate immune cell metabolism, that is not resolved by cholesterol lowering. We found that exposure to high cholesterol (HC) in vivo induced long-term metabolic changes in macrophages differentiated ex vivo from bone marrow, leading to a pro-inflammatory profile; these changes persisted in vivo despite cholesterol lowering. HSC from HC mice had altered chromatin accessibility that persisted after cholesterol lowering and was also present in bone marrow monocytes and tissue resident macrophages. HC provoked RUNX1-dependent downregulation of stearoyl-CoA desaturase (SCD) which reduced mono-unsaturated fatty acid (MUFA) availability for OxPhos in murine and human monocytes and macrophages. Supplementation with MUFA restored OxPhos capacity and promoted a shift towards a less pro-inflammatory macrophage phenotype in HC-trained BMDM. Bone marrow chimera and lineage tracking studies revealed that prior exposure of HSC to HC conferred adverse systemic metabolic effects on normocholesterolemic mice, with increased adipose tissue mass and increased migration of macrophages derived from HC-exposed HSC into adipose tissue, resulting in increased adipose tissue inflammation and systemic glucose intolerance. These findings indicate that HC results in long-lasting immuno-epigenetic memory in HSC which is refractory to lipid lowering, and provide strong evidence that exposure to HC can have prolonged consequences that require new therapeutic approaches, beyond cholesterol lowering.",
  "authors": [
    {
      "affiliations": [
        "University of Oxford"
      ],
      "name": "Gareth SD Purvis"
    },
    {
      "affiliations": [
        "University of Oxford"
      ],
      "name": "Jiahao Jiang"
    },
    {
      "affiliations": [
        "University of Oxford"
      ],
      "name": "Thomas K Hiron"
    },
    {
      "affiliations": [
        "University of Oxford"
      ],
      "name": "Christina Simoglou Karali"
    },
    {
      "affiliations": [
        "University of Oxford"
      ],
      "name": "Matthew Baxter"
    },
    {
      "affiliations": [
        "University of Oxford"
      ],
      "name": "Eileen McNeill"
    },
    {
      "affiliations": [
        "University of Oxford"
      ],
      "name": "Rohit Vijjhalwar"
    },
    {
      "affiliations": [
        "University of Oxford"
      ],
      "name": "Adam Meade"
    },
    {
      "affiliations": [
        "University of Oxford"
      ],
      "name": "David R Greaves"
    },
    {
      "affiliations": [
        "University of Oxford"
      ],
      "name": "Chris O’Callaghan"
    },
    {
      "affiliations": [
        "University of Oxford"
      ],
      "name": "Keith M Channon"
    }
  ],
  "title": "P19  Hypercholesterolaemia promotes epigenetic memory in hematopoietic stem cells that persists after lipid lowering and causes systemic immunometabolic dysfunction mediated by macrophage metabolic reprogramming",
  "uid": "deab80d6-b42c-5cb1-bef4-4114ee78746d"
}