{
  "abstract": "Background Streptococcus anginosus has been linked with an increasing risk of gastric cancer (GC) and recognised as a signature for GC screening.Objective To investigate the promotional effect of S. anginosus in terms of its metabolic interactions with the host.Design We used the functional profiles of shotgun metagenomic sequencing from stools to detect bioactive molecules relevant to S. anginosus. In vivo and in vitro experiments were used to validate the facilitation of S. anginosus to GC progression. S. anginosus clinical strains were isolated and cultivated from cancerous tissues to verify its promotion of GC via methionine production. S. anginosus ΔmetE mutant strains were constructed to confirm the critical role of metE in methionine biosynthesis.Results We verified S. anginosus facilitated GC progression in vivo and in vitro. Our functional analysis of metagenomes revealed a significant enrichment of bacterial methionine biosynthesis pathways in GC patients with high S. anginosus abundance. Methionine, identified here as one of the primary microbial metabolites derived from S. anginosus, contributed to GC progression in humans and mice. S. anginosus strains from cancerous tissues were found to promote GC via methionine production. We further observed a higher abundance and prevalence of metE gene in cancer stool metagenomes. By constructing an S. anginosus ΔmetE mutant strain, we confirmed the critical role of metE in methionine biosynthesis.Conclusion Our results elucidate the role of S. anginosus-derived methionine in GC progression, shedding light on intricate metabolic interplay between S. anginosus and host.",
  "authors": [
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Cheng-Bei Zhou"
    },
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Li-Cong Zhao"
    },
    {
      "affiliations": [
        "BGI Genomics, Shenzhen, China",
        "State Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen, China"
      ],
      "name": "Youwen Qin"
    },
    {
      "affiliations": [
        "Department of Infectious Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China"
      ],
      "name": "Jingchen Yu"
    },
    {
      "affiliations": [
        "State Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen, China"
      ],
      "name": "Wenxi Li"
    },
    {
      "affiliations": [
        "BGI Genomics, Shenzhen, China",
        "School of Biological Sciences & Medical Engineering, Southeast University, Nanjing, China"
      ],
      "name": "Qianhui Feng"
    },
    {
      "affiliations": [
        "State Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen, China"
      ],
      "name": "Xin Tong"
    },
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Resalaiti Abuduaini"
    },
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China",
        "Department of Gastroenterology, Secondary Affiliated Hospital of Zhejiang University, Hangzhou, China"
      ],
      "name": "Shi-Yuan Lu"
    },
    {
      "affiliations": [
        "Laboratory of Bacterial Pathogenesis, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China"
      ],
      "name": "Huang Tang"
    },
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Ya-Xuan Zhang"
    },
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Yun Cui"
    },
    {
      "affiliations": [
        "State Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen, China"
      ],
      "name": "Liang Xiao"
    },
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Lin-Hong Song"
    },
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Le-Kai Ni"
    },
    {
      "affiliations": [
        "BGI Genomics, Shenzhen, China"
      ],
      "name": "Kui Wu"
    },
    {
      "affiliations": [
        "BGI Genomics, Shenzhen, China"
      ],
      "name": "Huanzi Zhong"
    },
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Yi-Chao Jiang"
    },
    {
      "affiliations": [
        "State Key Laboratory of Genome and Multi-omics Technologies, BGI Research, Shenzhen, China"
      ],
      "name": "Yuanqiang Zou"
    },
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Xiao-Xu Leng"
    },
    {
      "affiliations": [
        "Department of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"
      ],
      "name": "Ming Wang"
    },
    {
      "affiliations": [
        "Department of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"
      ],
      "name": "Wen-Yi Zhao"
    },
    {
      "affiliations": [
        "Department of Gastrointestinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"
      ],
      "name": "Chao-Jie Wang"
    },
    {
      "affiliations": [
        "Department of Pathology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Qiang Liu"
    },
    {
      "affiliations": [
        "Department of Pharmacy, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China"
      ],
      "name": "Jia-Qi Zhang"
    },
    {
      "affiliations": [
        "Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai, China"
      ],
      "name": "Cheng Hu"
    },
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Ying-Xuan Chen"
    },
    {
      "affiliations": [
        "Laboratory of Bacterial Pathogenesis, Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China"
      ],
      "name": "Yu-Feng Yao"
    },
    {
      "affiliations": [
        "BGI Genomics, Shenzhen, China"
      ],
      "name": "Shida Zhu"
    },
    {
      "affiliations": [
        "Division of Gastroenterology and Hepatology",
        "Shanghai Institute of Digestive Disease",
        "NHC Key Laboratory of Digestive Diseases",
        "State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
      ],
      "name": "Jing-Yuan Fang"
    }
  ],
  "title": "Streptococcus anginosus-derived methionine promotes gastric cancer progression",
  "uid": "e45aaeaf-915b-5a0d-8a6a-0b36d9111c72"
}