{
  "abstract": "Background Dysosmobacter welbionis is a recently discovered butyrate producer whose presence in stool correlates with improved metabolic health. Whether its abundance is reduced in individuals with metabolic dysfunction-associated steatotic liver disease (MASLD) remains unknown. Mechanistic insight into its butyrate production from myo-inositol, a dietary compound from fruits, beans, grains and nuts with metabolic benefits, is also limited.Objective To assess population-level distribution, relative abundance and strain diversity of D. welbionis in humans, and to elucidate its metabolic capacity to ferment myo-inositol into butyrate.Design We analysed several human cohorts for associations with liver health and evaluated D. welbionis J115T supplementation in a diet-induced steatosis mouse model. An antibody-guided anaerobic cell-sorting strategy enabled isolation of distinct strains. We combined 13C-labelled inositol isotopes with NMR, mass spectrometry, genomics and proteomics.Results We found that D. welbionis and two related species (D. hominis and D. segnis) are prevalent gut bacteria in the human gut. D. welbionis abundance was reduced in MASLD across two cohorts and inversely correlated with fibrosis score in a third cohort. Treatment with D. welbionis J115T improved glycaemia and hepatic steatosis in high-fat diet fed mice. We identified a non-canonical myo-inositol-to-butyrate fermentation pathway. 19 human strains were isolated, comparative genomics of 23 strains revealed an open pangenome (about 2100 core genes) including the full myo-inositol fermentation pathway.Conclusion D. welbionis possesses a unique, conserved route to convert dietary myo-inositol into butyrate, distinguishing it from other commensals and supporting its potential as a next-generation probiotic for metabolic and liver health.",
  "authors": [
    {
      "affiliations": [
        "Louvain Drug Research Institute (LDRI), Metabolism and Nutrition research group (MNUT), UCLouvain, Université catholique de Louvain, Brussels, Belgium",
        "Walloon Excellence in LifeSciences and BIOtechnology (WELBIO) department, WELResearch Institute, Wavre, Belgium"
      ],
      "name": "Chi-Hsien Lee"
    },
    {
      "affiliations": [
        "Departments of Experimental Vascular Medicine, Amsterdam University Medical Centres (AMC), Amsterdam, Netherlands"
      ],
      "name": "Thi Phuong Nam Bui"
    },
    {
      "affiliations": [
        "Louvain Drug Research Institute (LDRI), Metabolism and Nutrition research group (MNUT), UCLouvain, Université catholique de Louvain, Brussels, Belgium",
        "Walloon Excellence in LifeSciences and BIOtechnology (WELBIO) department, WELResearch Institute, Wavre, Belgium"
      ],
      "name": "Camille Petitfils"
    },
    {
      "affiliations": [
        "Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland"
      ],
      "name": "Ching Jian"
    },
    {
      "affiliations": [
        "Louvain Drug Research Institute (LDRI), Metabolism and Nutrition research group (MNUT), UCLouvain, Université catholique de Louvain, Brussels, Belgium",
        "Walloon Excellence in LifeSciences and BIOtechnology (WELBIO) department, WELResearch Institute, Wavre, Belgium"
      ],
      "name": "Giselle C Wong"
    },
    {
      "affiliations": [
        "Louvain Drug Research Institute (LDRI), Metabolism and Nutrition research group (MNUT), UCLouvain, Université catholique de Louvain, Brussels, Belgium",
        "Walloon Excellence in LifeSciences and BIOtechnology (WELBIO) department, WELResearch Institute, Wavre, Belgium"
      ],
      "name": "Anthony Puel"
    },
    {
      "affiliations": [
        "Nutrition and obesities : systemic approaches, Nutriomics, Sorbonne Université, Inserm, Paris, France"
      ],
      "name": "Tiphaine Le Roy"
    },
    {
      "affiliations": [
        "BIOASTER, Lyon, France"
      ],
      "name": "Samuel Bellais"
    },
    {
      "affiliations": [
        "BIOASTER, Lyon, France"
      ],
      "name": "Bouthaina Ben Abdallah"
    },
    {
      "affiliations": [
        "BIOASTER, Lyon, France"
      ],
      "name": "Mélanie Nehlich"
    },
    {
      "affiliations": [
        "BIOASTER, Lyon, France"
      ],
      "name": "Thomas Leicht"
    },
    {
      "affiliations": [
        "Section of Biomolecular Medicine, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK"
      ],
      "name": "Manyi Jia"
    },
    {
      "affiliations": [
        "Department of Biosciences, School of Science & Technology, Nottingham Trent University, Nottingham, UK"
      ],
      "name": "Lesley Hoyles"
    },
    {
      "affiliations": [
        "IRCCS Neuromed, Pozzilli, Italy",
        "Department of Systems Medicine, Center for Atherosclerosis, University of Rome Tor Vergata, Rome, Italy"
      ],
      "name": "Massimo Federici"
    },
    {
      "affiliations": [
        "Department of Medical Sciences, School of Medicine, University of Girona, Girona, Spain",
        "CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain",
        "Department of Diabetes, Endocrinology and Nutrition, Dr. Josep Trueta University Hospital, Girona, Spain"
      ],
      "name": "Jose Manuel Fernández-Real"
    },
    {
      "affiliations": [
        "UMR 1297 INSERM, Team INCOMM, Rangueil Hospital, Toulouse, France"
      ],
      "name": "Remy Burcelin"
    },
    {
      "affiliations": [
        "Section of Biomolecular Medicine, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK",
        "European Genomic Institute for Diabetes, U1283 INSERM, UMR8199 CNRS, Institut Pasteur de Lille, Lille University Hospital, University of Lille, Lille, France"
      ],
      "name": "Marc-Emmanuel Dumas"
    },
    {
      "affiliations": [
        "Louvain Drug Research Institute (LDRI), Metabolism and Nutrition research group (MNUT), UCLouvain, Université catholique de Louvain, Brussels, Belgium"
      ],
      "name": "Nathalie M Delzenne"
    },
    {
      "affiliations": [
        "Functional Microbiome Research Group, Institute of Medical Microbiology, University Hospital of RWTH Aachen, Aachen, Germany"
      ],
      "name": "Thomas Clavel"
    },
    {
      "affiliations": [
        "Biochemistry, Wageningen University, Wageningen, Netherlands"
      ],
      "name": "Sjef Boeren"
    },
    {
      "affiliations": [
        "Proteomics, Metabolomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, Portici, Italy"
      ],
      "name": "Antonio Dario Troise"
    },
    {
      "affiliations": [
        "Proteomics, Metabolomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, Portici, Italy"
      ],
      "name": "Andrea Scaloni"
    },
    {
      "affiliations": [
        "Bioanalysis and Pharmacology of Bioactive Lipids Research Group (BPBL), Louvain Drug Research Institute (LDRI), UCLouvain, Université catholique de Louvain, Brussels, Belgium"
      ],
      "name": "Giulio G Muccioli"
    },
    {
      "affiliations": [
        "Departments of Experimental Vascular Medicine, Amsterdam University Medical Centres (AMC), Amsterdam, Netherlands",
        "Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland",
        "Laboratory of Microbiology, Wageningen University, Wageningen, Netherlands"
      ],
      "name": "Willem M De Vos"
    },
    {
      "affiliations": [
        "Louvain Drug Research Institute (LDRI), Metabolism and Nutrition research group (MNUT), UCLouvain, Université catholique de Louvain, Brussels, Belgium",
        "Walloon Excellence in LifeSciences and BIOtechnology (WELBIO) department, WELResearch Institute, Wavre, Belgium"
      ],
      "name": "Matthias Van Hul"
    },
    {
      "affiliations": [
        "Louvain Drug Research Institute (LDRI), Metabolism and Nutrition research group (MNUT), UCLouvain, Université catholique de Louvain, Brussels, Belgium",
        "Walloon Excellence in LifeSciences and BIOtechnology (WELBIO) department, WELResearch Institute, Wavre, Belgium",
        "Section of Biomolecular Medicine, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK",
        "Institute of Experimental and Clinical Research (IREC), UCLouvain, Université catholique de Louvain, Brussels, Belgium"
      ],
      "name": "Patrice D Cani"
    }
  ],
  "title": "Novel myo-inositol to butyrate fermentation pathway in the prevalent human gut species Dysosmobacter welbionis, a bacterium associated with improved metabolic and liver health",
  "uid": "7e2c90c4-da0b-5e62-9f5e-e3becfb1f712"
}