{
  "abstract": "There is limited understanding of the aetiology and disease progression of children with eosinophilic oesophagitis (EoE). Repeat endoscopies are required to monitor treatment response. In the BioEvocs study we will investigate volatile organic compounds (VOCs metabolome) and composition of the gut microbiome from stool samples in EoE patients and controls.As part of the BioEvocs study, we assessed demographics and nutritional characteristics in children with EoE and controls who provided stool samples for subsequent analysis.Over a period of 9.5 months, we obtained stool samples from 44 eligible EoE patients and 27 EoE follow-up patients, and samples from 30 non-EoE oesophageal control (OCB) patients and 30 normal control patients (NCB) from 4 sites in the UK. Patients between 3-17 years were eligible for the study. Normal controls were recruited from ophthalmology clinics. Mean age was 11.2 years (SD 4.0) for EoE patients with 80.7% male, 11.1 years for non-EoE oesophageal controls (SD 3.9) (n.s. p=0.99 compared to EoE) with 77.4% male, and normal controls 5.2 years (SD 1.9) (p-value<0.0001 compared to EoE and non-EoE controls, using Tukey’s HSD test with ANOVA), with 56% male.Histology was the gold standard and endoscopy data (6 questions (Q)). EoE was defined as in remission when eosinophils (eos) were <15/high power field (HPF), mild activity when eos 15-50/HPF, moderate when 51-100 eos/HPF and severe when eos >100/HPF. Clinical (50 Q) and nutritional (14 Q) questionnaires and PEESS scores (20 Q) were collected, as supplementary information to stool samples (4Q).PEESS scores (0 indicating no symptoms and 80 maximal oesophageal-associated symptoms) were completed by parents in EOB, EOF and OCB patients. Response type was defined by decreasing activity by one or more levels. and non-response as increasing activity compared to baseline.Selected questions from 94 variables:Processed food: Does your child eat daily one or more portions of packaged foodBreastfeeding: Exclusively breast fed over 3 monthsNeurodiversity: Diagnosed or assessed with autism, ASD, ADHD, sensory processing disorder, other neurodiversityIn this pilot study, patients with EoE and non-EoE oesophageal controls showed similar age, but older than normal controls. EoE patients were not statistically different in terms of neurodiversity, indicators of processed food, and duration of breastfeeding compared to controls. We will present detailed analysis from 94 variables and findings from the gut metabolome and microbiome which are currently being analysed.BSPGHAN/GutsUK/Dr Falk sponsored BioEvocs.Abstract OC58 Table 1Demographics at baseline and follow-up from patients who provided stool samplesPEESSpointsin mean (SD)Neurodi-verse (Y)proportion of patientsBreast-feeding (Y)proportion of patientsProcessed food (Y)proportion of patientsBase-lineEOEEOB (baseline)23.96 (16.78)18/48 (0.375)20/47 (0.425)35/47 (0.744)ControlsOCB Oesophageal28.66 (14.13)9/31 (0.290)9/31 (0.290)25/30 (0.833)NCB NormalN/A10/32 (0.312)9/32 (0.281)29/32 (0.906)Chi-SquareN/A0.69 (n.s.)2.3 (n.s.)3.4 (n.s.)Follow-UpEoEResponders17.61 (12.19)9/22 (0.409)8/22 (0.363)18/22 (0.818)Non-Responders20.85 (18.58)8/14 (0.571)6/14 (0.428)10/14 (0.714)Chi-SquareN/A0.9 (n.s.)0.01 (n.s.)0.5 (n.s.)",
  "authors": [
    {
      "affiliations": [
        "Alder Hey Children’s NHS Foundation Trust",
        "University of Liverpool"
      ],
      "name": "Marcus KH Auth"
    },
    {
      "affiliations": [
        "University of Liverpool"
      ],
      "name": "Rachael Slater"
    },
    {
      "affiliations": [
        "Alder Hey Children’s NHS Foundation Trust"
      ],
      "name": "Raj Parmar"
    },
    {
      "affiliations": [
        "Great Ormond Street Hospital NHS Foundation Trust"
      ],
      "name": "Edward Gaynor"
    },
    {
      "affiliations": [
        "Alder Hey Children’s NHS Foundation Trust"
      ],
      "name": "Damien Yeo"
    },
    {
      "affiliations": [
        "Chelsea and Westminster Hospital NHS Foundation Trust"
      ],
      "name": "Jenny Epstein"
    },
    {
      "affiliations": [
        "Sheffield Chiildren’s Hospital NHS Foundation Trust"
      ],
      "name": "Shishu Sharma"
    },
    {
      "affiliations": [
        "Alder Hey Children’s NHS Foundation Trust"
      ],
      "name": "Will Simmons"
    },
    {
      "affiliations": [
        "Alder Hey Children’s NHS Foundation Trust"
      ],
      "name": "Rajeev Shukla"
    },
    {
      "affiliations": [
        "Alder Hey Children’s NHS Foundation Trust"
      ],
      "name": "Michael Staunton"
    },
    {
      "affiliations": [
        "Alder Hey Children’s NHS Foundation Trust"
      ],
      "name": "Daniel Hurrell"
    },
    {
      "affiliations": [
        "Alder Hey Children’s NHS Foundation Trust"
      ],
      "name": "Alexandra Patrick"
    },
    {
      "affiliations": [
        "Alder Hey Children’s NHS Foundation Trust"
      ],
      "name": "Ela Owen"
    },
    {
      "affiliations": [
        "Alder Hey Children’s NHS Foundation Trust"
      ],
      "name": "Stella Ng"
    },
    {
      "affiliations": [
        "University of Liverpool"
      ],
      "name": "Chris Probert"
    }
  ],
  "title": "OC58 Clinical and nutritional characteristics of children with eosinophilic oesophagitis (EoE) in the multi-centre BioEvocs study",
  "uid": "8270ebfe-7369-5340-a641-3e9728a5b283"
}