{
  "abstract": "Background While cardiopulmonary transition in neonates is well described, molecular changes which accompany it are not. The Extracellular Vesicles in Early preterm neonates and Thrombin generation (EVENT) study sought to explore molecular transition in preterm birth. How this differs from term-born neonates is not well described.Methods A post hoc evaluation of proteomic data generated from a subset of the prospective, single site observational EVENT study was performed comparing extracellular vesicle enriched plasma from term neonates (n=10) with preterm neonates (n=20) using a mass spectrometry, data dependent acquisition-based proteomic approach. Bioinformatics was performed using MaxQuant proteomics software and R statistical software.Results Substantial differences were found between the preterm and term extracellular vesicle proteome, with proteins involved in extracellular matrix biology, immune function and embryogenesis all differentially expressed. Preterm neonates could be identified by their proteomic profile alone, reflecting their distinct physiology.Conclusions These protein expression differences may underlie the pathogenesis of complications of prematurity, such as bronchopulmonary dysplasia, retinopathy of prematurity and intraventricular haemorrhage.",
  "authors": [
    {
      "affiliations": [
        "Paediatrics, Rotunda Hospital, Dublin, Ireland",
        "Conway Institute, University College Dublin, Dublin, Ireland"
      ],
      "name": "Daniel O’Reilly"
    },
    {
      "affiliations": [
        "Department of Neonatology, Chelsea and Westminster Hospital NHS Foundation Trust, London, UK"
      ],
      "name": "Claire A Murphy"
    },
    {
      "affiliations": [
        "Conway Institute, University College Dublin, Dublin, Ireland",
        "Center for Interdisciplinary Cardiovascular sciences, Harvard Medical School, Boston, Massachusetts, USA"
      ],
      "name": "Luisa Weiss"
    },
    {
      "affiliations": [
        "Conway Institute, University College Dublin, Dublin, Ireland"
      ],
      "name": "Hayley Macleod"
    },
    {
      "affiliations": [
        "Conway Institute, University College Dublin, Dublin, Ireland"
      ],
      "name": "Ana Le Chevillier"
    },
    {
      "affiliations": [
        "Conway Institute, University College Dublin, Dublin, Ireland",
        "Department of Neonatology, Liverpool Women’s Hospital, Liverpool, UK"
      ],
      "name": "Elaine Neary"
    },
    {
      "affiliations": [
        "Department of Laboratory Medicine, Rotunda Hospital, Dublin, Ireland"
      ],
      "name": "John O’Loughlin"
    },
    {
      "affiliations": [
        "Department of Paediatrics, Royal College of Surgeons in Ireland, Dublin, Ireland",
        "Department of Neonatology, The Rotunda Hospital, Dublin, Ireland"
      ],
      "name": "Afif EL-Khuffash"
    },
    {
      "affiliations": [
        "Conway Institute, University College Dublin, Dublin, Ireland",
        "Department of Haematology, Mater Misericordiae University Hospital, Dublin, Ireland"
      ],
      "name": "Barry Kevane"
    },
    {
      "affiliations": [
        "Haematology, Rotunda, Dublin, Ireland"
      ],
      "name": "Fionnuala Ni Ainle"
    },
    {
      "affiliations": [
        "Neonatology, Rotunda Hospital, Dublin, Ireland"
      ],
      "name": "Naomi McCallion"
    },
    {
      "affiliations": [
        "Conway Institute, University College Dublin, Dublin, Ireland",
        "Insight Research Ireland Centre for Data Analytics, University College Dublin, Dublin, Ireland"
      ],
      "name": "Patricia B Maguire"
    }
  ],
  "title": "Comparing extracellular vesicle enriched plasma proteomes between term and preterm neonates over the first days of life: post hoc analysis of a prospective observational study",
  "uid": "f029dbcd-35d5-561c-a420-4c75b15af348"
}