{
  "abstract": "Purpose To report the feasibility of unaided direct visualisation of an orientation (‘F’) mark on Descemet membrane endothelial keratoplasty (DMEK) grafts immediately after surgery and on postoperative day 1, as an adjunct for early confirmation of graft orientation.Methods This observational case series included 50 eyes undergoing standard DMEK using donor tissue marked with gentian violet dye applied as an ‘F’ orientation mark. Unaided visualisation (observation without a magnification aid such as a slit-lamp or operating microscope) was assessed intraoperatively and on postoperative day 1. Observations were documented photographically. Viewing conditions, lighting and pupil status were standardised. No adjustments to surgical technique or postoperative management were made. Consent was obtained for photography.Results In a subset of eyes, the ‘F’ orientation mark was visible to the operating surgeon with the unaided eye immediately after surgery and to the examining clinician without magnification on postoperative day 1. Visibility permitted confirmation of correct graft orientation without the use of slit-lamp biomicroscopy or intraoperative OCT. No adverse events were attributable to graft marking were observed, and graft attachment was comparable to standard cases.Conclusions Unaided visualisation of an ‘F’ orientation mark may be possible in selected cases immediately after DMEK and on postoperative day 1. Whilst not a replacement for established intraoperative or postoperative assessment methods, this observation may serve as a simple adjunct for early graft orientation confirmation and may be uploaded to EPR for proof of the same.",
  "authors": [
    {
      "affiliations": [
        "Luton and Dunstable Hospital, Luton, UK"
      ],
      "name": "Poonam Sharma"
    },
    {
      "affiliations": [
        "Luton and Dunstable Hospital, Luton, UK"
      ],
      "name": "Tejal Patel"
    },
    {
      "affiliations": [
        "OCL Vision, London, UK"
      ],
      "name": "Allon Barsam"
    }
  ],
  "title": "P8 Early postoperative unaided visualisation of an orientation mark after DMEK",
  "uid": "5af4f4d4-f491-5875-924f-522259194dba"
}
