{
  "abstract": "The monolayer of approximately 300,000 human corneal endothelial cells (hCECs) on the posterior surface of the cornea is essential to maintain transparency but is non-self-regenerative. Corneal blindness can currently only be treated by corneal transplantation, hindered by a global donor shortage, highlighting the need for developing tissue and/or cell therapy. The mass production of these advanced therapy medicinal products requires obtaining high-yield, high-quality endothelial cell culture characterized by hexagonal shape, low size variability, and high endothelial cell density (ECD). Among the usual critical quality attributes that combine the expression of differentiation markers, ECD and cell morphology parameters, the latter are not optimally measured in vitro by conventional image analysis which poorly recognize adherent cultured cells. We developed a high-performance automated segmentation using Cellpose algorithm and an original analysis method, improving calculation of classical morphological parameters (coefficient of variation of cell area and hexagonality) and introducing new parameters specific to hCECs culture in vitro. Considering the importance of the extracellular matrix in vivo, and the panel of molecules available for coating cell culture plastics, we used these new tools to perform a comprehensive comparison of 13 molecules (laminins and collagens). We demonstrated their ability to discriminate subtle differences between cultures.",
  "authors": [
    {
      "affiliations": [
        "Biology, Engineering and Imaging for Ophthalmology, EA2521, Faculty of Medicine, Jean Monnet University, Saint Etienne, France"
      ],
      "name": "Zhiguo He"
    },
    {
      "affiliations": [
        "Biology, Engineering and Imaging for Ophthalmology, EA2521, Faculty of Medicine, Jean Monnet University, Saint Etienne, France"
      ],
      "name": "Louise Parveau"
    },
    {
      "affiliations": [
        "Biology, Engineering and Imaging for Ophthalmology, EA2521, Faculty of Medicine, Jean Monnet University, Saint Etienne, France"
      ],
      "name": "Gauthier Travers"
    },
    {
      "affiliations": [
        "Biology, Engineering and Imaging for Ophthalmology, EA2521, Faculty of Medicine, Jean Monnet University, Saint Etienne, France"
      ],
      "name": "Corantin Maurin"
    },
    {
      "affiliations": [
        "Biology, Engineering and Imaging for Ophthalmology, EA2521, Faculty of Medicine, Jean Monnet University, Saint Etienne, France"
      ],
      "name": "Guillaume Bonnet"
    },
    {
      "affiliations": [
        "Biology, Engineering and Imaging for Ophthalmology, EA2521, Faculty of Medicine, Jean Monnet University, Saint Etienne, France"
      ],
      "name": "Inès Aouimeur"
    },
    {
      "affiliations": [
        "Biology, Engineering and Imaging for Ophthalmology, EA2521, Faculty of Medicine, Jean Monnet University, Saint Etienne, France",
        "Department of Ophthalmology, University Hospital, Saint Etienne, France"
      ],
      "name": "Philippe Gain"
    },
    {
      "affiliations": [
        "Biology, Engineering and Imaging for Ophthalmology, EA2521, Faculty of Medicine, Jean Monnet University, Saint Etienne, France",
        "Department of Ophthalmology, University Hospital, Saint Etienne, France"
      ],
      "name": "Gilles Thuret"
    }
  ],
  "title": "P.11. Investigating the role of molecular coating in human corneal endothelial cell primary culture",
  "uid": "959182d7-9361-58b0-9ef9-ed4e04e10ede"
}